The area measurements are also recorded in tabular form,Īnd can be displayed (Show Results) printed (Print) or exported (Export) to a spreadsheet. Option-click with the text tool to automatically label the peaks, in reverse order,.Measure the areas of the peaks by clicking inside each one in succession with the.Note that you can hold the shift key down to constrain Use the line drawing tool to draw base lines and drop lines so that each peak definesĪ closed area as shown above.Move the rectangular selection (by clicking inside it and dragging) and outline (using.A copy of the image will be displayed with the first lane outlined. Lane for vertically oriented lanes and the top lane for horizontal lanes. Use the rectangular selection tool to outline the first lane.To open the file 'Gel Plotting Macros' in the Macros folder. Failure to do this could result in incorrect and misleading measurements.Īre not shown in the Special menu then use Load Macros Use the Calibrate command to calibrate the image to a calibrated optical density.With known concentrations or by comparing with results obtained using a densitometer. Obtained using this procedure should not be trusted without testing using standards On different gels unless the gels are calibrated to known standards. Note that this technique cannot be used to compare bands It also demonstrates some of theĪnd also a few shortcuts. To analyze a one-dimensional electrophoretic gel. The following is one possible procedure for using Image

Imagej western blot quantification